Studies on Milk Xanthine Oxidase
نویسندگان
چکیده
منابع مشابه
The composition of milk xanthine oxidase.
The composition of milk xanthine oxidase has been reinvestigated. When the enzyme is prepared by methods that include a selective denaturation step in the presence of sodium salicylate the product is obtained very conveniently and in high yield, and is homogeneous in the ultracentrifuge and in recycling gel filtration. It has specific activity higher than previously reported preparations of the...
متن کاملInhibitory Effect of Ruta graveolens L. Extract on Guinea Pig Liver and Bovine Milk Xanthine Oxidase
Flavonoids could serve as potent inhibitors of xanthine oxidase (XO). In the present study, the effects of Ruta graveolens L. extract and its major isolated flavonoids, quercetin and rutin, on guinea pig liver XO have been investigated. The inhibitory effects of R. graveolens, quercetin and its glycoside form, rutin, were assayed spectrophotometrically. R. graveolens...
متن کاملSpectroscopic studies on the iron-sulfur centers of milk xanthine oxidase.
The optical electron paramagnetic resonance and Mössbauer spectral properties of the two iron-sulfur centers present in milk xanthine oxidase have been reexamined. It is found in the case of the optical spectral change observed on reduction of the enzyme that the two centers contribute approximately equally, with a ratio of spectral contributions for Fe/S I and Fe/S II of 0.55:0.45. This conclu...
متن کاملElectron paramagnetic resonance and circular dichroism studies on milk xanthine oxidase.
The g = 1.94 electron paramagnetic resonance signal of xanthine oxidase intensifies rapidly below 77” I< and is found to resemble markedly that of spinach ferredoxin. At very low temperatures a new resonance is observed in the reduced protein at g = 2.11 and tentatively ascribed to a second type of iron chromophore. The time dependence of flavin and molybdenum during titration with substrate is...
متن کاملFurther studies with xanthine oxidase inhibitors.
The osidase and dehydrogenase activities in the enzymes oxidizing xanthine can be differentiated by the use of certain inhibitors (1). The present study was designed to see if such inhibitors are specific for the reaction with oxygen, or whether these agents also block the transfer of electrons to cytochrome c and nitrate. Both reduced diphosphopyridine nucleotide and hypoxanthine were studied ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91738-2